Clinton H. Howard and Kimberly Lloyd Royal BodyCare is conducting an ongoing series of laboratory and clinical studies designed to evaluate the nutritional characteristics and benefits of Microhydrin (250 mg per capsule) as a mineral antioxidant. The following is a brief summary of the results of studies completed as of July 30, 1999. Microhydrin in Intra and Extra Cellular Hydration A double blind placebo controlled pilot study was conducted using the RJL Bioelectrical Impedance Analyzer developed by R. J. Liedtke. Seven subjects received 4 capsules of Microhydrin per day for two weeks and were crossed over receiving 4 capsules per day of a placebo (rice bran flour). Averaged values showed increased intracellular and extracellular hydration due to the consumption of Microhydrin as compared to the placebo group. When consuming Microhydrin, the value for Total Body Water (intracellular and extracellular water) increased by 2.7%. The changes observed in TBW when subjects consumed Microhydrin, compared to the placebo, showed statistical significance (p < 0.05) using a student’s test for small sample groups.
Body Cell Mass (BCM) and Intracellular Water (ICW) are assessments of intracellular volume and water inside the cell, respectively. Extracellular Body Water, water that bathes the cells, also showed increased volume. Intracellular water makes up approximately 60% of the total body water of healthy adults. Intracellular water, as an indicator of cell integrity, is found to be higher in babies but decreases as adults age or lose body cell mass. Healthy tissue cells hold water within the cells and have higher anabolic (building up) function rather than catabolic (breaking down) function. Microhydrin® Increased Mitochondria Membrane Potential Using Fluorescence Microscopy Confocal fluorescence microscopy revealed that when cells were incubated with Microhydrin at a concentration of 200 ug/ml, approximately 10% of the mitochondria showed enhanced membrane potential. Normal cells show a membrane potential of 120-140 mV. However, after addition of Microhydrin to the cell culture media, 10% of the total mitochondria within the individual cells measured greater than 140 mV. This test measures changes in the electronic potential across the mitochondria membrane due to enhanced electron activity. These investigators are continuing to study the properties of Microhydrin towards enhancement of NADH and ATP production in various assays with fluorescent microscopy.
Microhydrin Protection
Against Oxidative Stress A double blind placebo controlled crossover pilot study was conducted on 7 subjects who received 4 capsules/day of Microhydrin for two weeks, and received 4 capsules/day of a placebo for two weeks. Urine alkenal/creatinine ratios were measured. During Microhydrin supplementation, a 43% increase in free radical protection was observed as compared to the placebo group. Microhydrin was observed in this study to protect against serum alkenals. Alkenals are the oxidative products of serum lipid peroxides occurring from free radical attacks on cellular lipid membranes and lipoproteins. They are indicators of free radical damage in the body associated with a higher risk of age related diseases. Evaluation of Microhydrin in the Production of NADH ATP production, the principal source of energy in the mitochondria, depends on the production of NADH. Reduced hydrogen, generated by the Kreb’s Cycle, is carried by NADH into the mitochondria for the electron transport chain reactions that will ultimately create a molecule of water and ATP. Hydrogen is one of the most important elements donating an electron, an electron pair or its proton to reduction/oxidation reactions of numerous enzymes and intermediates within the cell’s metabolic pathways. In vivo assays measured the direct conversion of NAD+ to
NADH by the addition of Microhydrin. The sequential addition of increasing concentrations of Microhydrin to NAD+ showed a linear increase in NADH production as measured by its absorption at 350 nm. Copyright© 1999 Royal BodyCare, Inc. All Rights Reserved. |
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